Real-time, continuous monitoring of biomarkers to advance soldier performance and readiness

Introduction: Established methods for monitoring specific molecules in the living body, such as continuous glucose monitor, rely on enzymatic or chemical reactivity of their targets, and thus are not generalizable to arbitrary new targets. For this reason, only a handful metabolites neurotransmitters, no hormones protein biomarkers, can currently be measured vivo1. Against background, we developing molecular measurement platform that: (1) has been demonstrated able work body (2) is independent its targets general. This technology will allow real-time biomarkers warfighters field. Methods: Our approach uses electrochemical aptamer-based (EAB) sensors. Aptamers synthetic nucleic acids generated by an in-vitro selection scheme selectively bind The aptamers engineered undergo large-scale conformational change upon binding target. By attaching one end aptamer interrogating electrode modifying other with redox reporter, target easily monitored using standard techniques. Because every step process rapidly reversible, EAB sensors monitor rising falling concentrations vivo real-time. Results: Using 75-micron diameter, 6-millimeter long have multiple analytes places intravenously subcutaneously2,3,4,5. achieve clinically relevant precision accuracy, time resolution within seconds3. Moving forward, adapting pain-free microneedles wearable, wireless electronics create convenient, status humans Discussion/conclusions: It now possible wide range markers seconds convenient wearable technology. References 1Kim J, Campbell AS, de Ávila B, et al. Wearable biosensors healthcare monitoring. Nat Biotechnol 2019; 37(4):389–406. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8183422/ 2Arroyo-Curras N, Somerson Vieira P, Real-time small awake, ambulatory animals. Proc Natl Acad Sci USA 2017; 114:645–650. https://doi.org/10.1073/pnas.1613458114 3Idili A, Gerson Kippin T, Seconds-resolved, situ measurements plasma phenylalanine disposition rats. Anal Chem 2021; 93:4023-4032. https://doi.org/10.1021/acs.analchem.0c05024 4Idili Arroyo-Curras Ploense KL, Seconds-resolved pharmacokinetic chemotherapeutic irinotecan body. 10:8164-8170. https://doi.org/10.1039/C9SC01495K 5Dauphin-Ducharme Yang K, Electrochemical improved therapeutic drug high-precision, feedback-controlled delivery. ACS Sensors 4:2832-2835. https://doi.org/10.1021/acssensors.9b01616